Diagnosis of Hemophilia and Other
Bleeding Disorders: A Laboratory Manual
Second Edition, 2010
Steve Kitchen, Angus McCraw, Marión Echenagucia on behalf of the WFH Laboratory Sciences Committee
This WFH manual is the definitive how-to guide for the accurate diagnosis of hemophilia and other bleeding disorders. Written and reviewed by the world’s leading experts in coagulation testing, this comprehensive, revised edition features new sections on two-stage clotting and chromogenic FVIII assays, von Willebrand factor collagen binding assay and multimer analysis, and the use of coagulometers.
Click here to download the complete PDF, or select individual sections below.
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Laboratory Manual Demonstration Videos
NOW AVAILABLE: Step-by-step video demonstrations of the three most important manual diagnostic techniques:
- APTT (Section 13)
- One-stage FVIII assay (Section 23)
- FVIII inhibitor assay (Section 28)
Each video features:
- careful demonstration of correct technique
- detailed explanations of the background theory of the assay
- special considerations for issues of limited resources
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- plotting and interpretation of typical results
- searchable transcripts
- record sheets and graph paper to download
- discussion of the important reagents
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Access the videos here:
APTT
One-stage FVIII
Inhibitors
Contents
- Laboratory Equipment
- Checking Pipette and Weighing Balance Calibration
- Laboratory Safety
- Sample Collection and Pre-Analytical Variables
- Internal Quality Control and External Quality Assessment
- Manual Tilt-Tube Technique
- Preparation and Calibration of Pooled Normal Plasma (PNP)
- Establishing a Normal Reference Range
- Reagents
- Platelet Count
- Bleeding Time
- Prothrombin Time (PT)
- Activated Partial Thromboplastin Time (APTT)
- Mixing Tests For Further Investigation of Abnormal PT and APTT
- Thrombin Clotting Time
- Thrombin Time in the Presence of Protamine Sulphate to Detect Presence of Heparin
- Reptilase Time
- Fibrinogen (Modifi ed Clauss Assay)
- Removal of Heparin from Plasma
- Fibrinogen Antigen Assay by Radial Immunodiffusion (RID)
- Factor XIII Screen
- Assays Based on Prothrombin Time (Factors II, V, VII, or X)
- Factor Assays Based on APTT (One-Stage Assay of FVIII:C, FIX, FXI, or FXII)
- Factor VIII:C Assays in Cryoprecipitate
- Two-Stage Clotting Assay for Factor VIII:C
25.1 Production of Combined Reagent for Two-Stage Factor FVIII:C Assay
- Chromogenic FVIII:C Assay
- One-Stage Intrinsic Assay of Prekallikrein (PKK) and High Molecular Weight Kininogen (HMWK)
- Clotting Factor Inhibitor Screen Based on APTT
- Ristocetin Cofactor Activity/von Willebrand Factor Activity (VWF: RCo or VWF:Act)
29.1 Fixed Platelet Preparation
- Von Willebrand Factor Antigen by Elisa (VWF:Ag)
- Von Willebrand Factor Collagen Binding Assay (VWF:CB)
- Factor VIII Binding Assay for Diagnosis of von Willebrand Disease Normandy
- VWF Multimer Analysis
- Quantitative Measurement of FVIII Inhibitors
- Factor IX Inhibitor Assay
- Factor XIII Activity Assay
- Lupus Anticoagulant and Anti-Phospholipid Antibodies
- Dilute Russell’s Viper Venom Time (DRVVT)
38.1 Preparation of Washed Platelets for DRVVT
- Platelet Function Testing
39.1 Clot Retraction
39.2 Measurement of Platelet Aggregation
- Lyophilization of Plasma
- Evaluation and Use of Coagulometers
- Molecular Genetic Analysis
Updated July 2016